AustralieFrV1, Main, Exploration, bibRecord, 008062

Synthesis of Nickel-Chelating Fluorinated Lipids for Protein Monolayer Crystallizations

Identifieur interne : 008062 ( Main/Exploration ); précédent : 008061; suivant : 008063

Synthesis of Nickel-Chelating Fluorinated Lipids for Protein Monolayer Crystallizations

Auteurs : Waleed M. Hussein [Australie] ; Benjamin P. Ross [Australie] ; Michael J. Landsberg [Australie] ; Daniel Levy [France] ; Ben Hankamer [Australie] ; Ross P. Mcgeary [Australie]

Source :

Journal of organic chemistry [ 0022-3263 ] ; 2009.

RBID : Pascal:09-0206214

Descripteurs français

Pascal (Inist)
- Synthèse chimique, Nickel, Fluor composé organique, Bacillus subtilis, Lipide, Protéine membranaire, Couche monomoléculaire, Cristallisation, Fonctionnalisation, Composé ramifié, Composé hydrophile, Interaction moléculaire, Alkylation, Alcool, Sonication, Catalyse transfert phase, Structure cristalline, Aminoacide, Hétérocycle azote, Histidine dérivé.
Wicri :
- topic : Nickel, Alcool.

English descriptors

KwdEn :
- Alcohol, Alkylation, Aminoacid, Bacillus subtilis, Branched compound, Chemical synthesis, Crystalline structure, Crystallization, Functionalization, Hydrophilic compound, Lipids, Membrane protein, Molecular interaction, Monolayer, Nickel, Nitrogen heterocycle, Organic fluorine compounds, Phase transfer catalysis, Sonication.

Abstract

Nickel-chelating lipids have been synthesized for use as functionalized templates for 2-D crystallization of membrane proteins. These monolayer-forming lipids have been designed with three distinct components: (i) a branched hydrocarbon tail to confer fluidity of the monolayer, (ii) a perfluorinated central core for detergent resistance, and (iii) a nickel-chelating hydrophilic headgroup to facilitate binding of recombinant, polyhistidine-tagged fusion proteins. Alkylations of fluorinated alcohols used in these syntheses proceed in good yields only with the application of prolonged sonication and, in some cases, in the presence of phase-transfer catalysts. Formation of 2-D crystals of the His-tagged membrane protein BmrA from Bacillus subtillis is reported.

Affiliations:

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Le document en format XML

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<term>Chemical synthesis</term>
<term>Crystalline structure</term>
<term>Crystallization</term>
<term>Functionalization</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Synthèse chimique</term>
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<term>Bacillus subtilis</term>
<term>Lipide</term>
<term>Protéine membranaire</term>
<term>Couche monomoléculaire</term>
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<front><div type="abstract" xml:lang="en">Nickel-chelating lipids have been synthesized for use as functionalized templates for 2-D crystallization of membrane proteins. These monolayer-forming lipids have been designed with three distinct components: (i) a branched hydrocarbon tail to confer fluidity of the monolayer, (ii) a perfluorinated central core for detergent resistance, and (iii) a nickel-chelating hydrophilic headgroup to facilitate binding of recombinant, polyhistidine-tagged fusion proteins. Alkylations of fluorinated alcohols used in these syntheses proceed in good yields only with the application of prolonged sonication and, in some cases, in the presence of phase-transfer catalysts. Formation of 2-D crystals of the His-tagged membrane protein BmrA from Bacillus subtillis is reported.</div>
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Synthesis of Nickel-Chelating Fluorinated Lipids for Protein Monolayer Crystallizations

Synthesis of Nickel-Chelating Fluorinated Lipids for Protein Monolayer Crystallizations

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri